219
Views
0
CrossRef citations to date
0
Altmetric
Research Article

Comparison of chemokine and receptor gene expression between Wharton's jelly and bone marrow-derived mesenchymal stromal cells

, , , , &
Pages 26-33 | Received 12 May 2011, Accepted 08 Jul 2011, Published online: 18 Nov 2011
 

Abstract

Background aims. Because of their multilineage differentiation capacity, immunomodulatory role and homing ability, mesenchymal stromal cells (MSC) are emerging as a new therapeutic strategy for treating a variety of disorders. Although bone marrow (BM) is the best characterized source of MSC, Wharton's jelly (WJ) of the umbilical cord holds great promise as an alternative. As delivery direct to the site of injury is not always feasible, efficient homing of MSC to the site of injury is critical for inducing tissue repair and regeneration. MSC express a wide variety of growth factors, chemokines and receptors that are important for cell migration, homing and re-establishment of blood supply for recovery of damaged tissues. Methods. Detailed chemokine and receptor gene expression profiles of WJ MSC were established, and subsequently compared with those of BM-derived MSC using a polymerase chain reaction (PCR) array. Secretion of growth factors was analyzed and evaluated using culture supernatant from WJ and BM MSC. Results. Our results revealed a differential expression pattern of the chemokines and their receptors between WJ- and BM-derived MSC. Several Glutamic acid-Leucine-Arginine; ELR-positive CXC chemokine genes and secretion of growth factors, which promote angiogenesis, were found to be up-regulated in WJ MSC. Conclusions. To understand better the localization and mechanism of tissue repair by transplanted WJ MSC, we attempted chemokine and their receptor transcription profiling, followed by analysis of growth factors secreted by WJ MSC, and compared them against those of BM MSC. The data suggest that MSC from different sources can be explored for distinct therapeutic roles.

Acknowledgments

This work was supported by internal funding of Stempeutics Research Pvt Ltd, Bangalore, India, Institute for Medical Research, Kuala Lumpur, Malaysia, and SERC Fast track grant from Department of Science and Technology (DST), India. We are grateful to Dr Praveena Shenoi, Consultant Obstetrician & Gynaecologist, Manipal Hospital, for generously providing the umbilical cord samples, and the donors for giving us their informed consent. We also thank Ms Lipsa Mohanty and Ms Usha Nekanti for their assistance in umbilical cord processing. SB and PV contributed equally to this work.

Disclosures: The authors declare no competing financial interests.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.