Studies on collagen-tannic acid-collagenase ternary system: Inhibition of collagenase against collagenolytic degradation of extracellular matrix component of collagen

Abstract

We report the detailed studies on the inhibitory effect of tannic acid (TA) on Clostridium histolyticum collagenase (ChC) activity against degradation of extracellular matrix component of collagen. The TA treated collagen exhibited 64% resistance against collagenolytic hydrolysis by ChC, whereas direct interaction of TA with ChC exhibited 99% inhibition against degradation of collagen and the inhibition was found to be concentration dependant. The kinetic inhibition of ChC has been deduced from the extent of hydrolysis of N-[3-(2-furyl) acryloyl]-Leu-Gly-Pro-Ala (FALGPA). This data provides a selective competitive mode of inhibition on ChC activity seems to be influenced strongly by the nature and structure of TA. TA showed inhibitor activity against the ChC by molecular docking method. This result demonstrated that TA containing digalloyl radical possess the ability to inhibit the ChC. The inhibition of ChC in gaining new insight into the mechanism of stabilization of collagen by TA is discussed.

Keywords::

• Extracellular matrix
• collagen
• collagenase
• collagenase inhibition
• tannic acid

Acknowledgment

The first author acknowledges the Council of Scientific & Industrial Research (CSIR), New Delhi, Government of India, for his CSIR-SRF fellowship. The assistance of S. Muthukumar, Research Scholar, Marine Bioinformatics Centre, National Institute of Ocean Technology, Chennai, for molecular docking work is acknowledged.

Declaration of interest

The authors declare no conflicts of interest.