In vitro efficacy of some cattle drugs on bovine serum paraoxonase 1 (PON1) activity

Abstract

Serum paraoxonase 1 (EC 3.1.8.1, PON1), a calcium-associated enzyme, has an ability to hydrolyze organophosphate compounds. Related to this property, PON1 has a critical role in antioxidant mechanisms. It is well-known that the enzyme protects LDL from oxidation. In this study we investigated the in vitro inhibitory effects of some drugs. These drugs are oxytocin, dexamethasone, atropine sulphate, gentamicin sulphate, sulfadoxine-trimethoprim, furosemid, metamizole sodium and toldimfos sodium. The IC₅₀ values obtained varied markedly from 0.014 to 507.72 mg/mL. According to our findings, most potent and significant inhibition was displayed by dexamethasone, atropine sulphate and furosemid.

Keywords::

• Paraoxonase
• inhibition
• oxytocin
• dexamethasone
• atropine sulphate
• gentamicin sulphate
• sulfadoxine-trimethoprim
• furosemid
• metamizole sodium
• toldimfos sodium

Acknowledgement

This work was carried out in the Balikesir University Research Center of Applied Sciences (BURCAS) Balikesir/Turkey.

Declaration of interest

The authors report no conflict of interest.