Antiproliferative activity of Humulus lupulus extracts on human hepatoma (Hep3B), colon (HT-29) cancer cells and proteases, tyrosinase, β-lactamase enzyme inhibition studies

Abstract

The aims of this study were to examine the antiproliferation of Humulus lupulus extracts on human hepatoma carcinoma (Hep3B) and human colon carcinoma (HT-29) cell lines along with enzyme inhibitory effects of the crude extracts. Potential cell cytotoxicity of six different H. lupulus extracts were assayed on various cancer cells using MTT assay at 24, 48 and 72 h intervals. Methanol-1 extract has inhibited the cell proliferation with doses of 0.6–1 mg/mL in a time dependent (48 and 72 hours) manner in Hep3B cells with 70% inhibition, while inhibitory effect was not seen in colon cancer cells. Acetone extract has increased the cell proliferation at low doses of 0.1 mg/mL for 72 h in Hep3B cells and 0.1–0.2 mg/mL for 48 and 72 h in HT29 cells. The inhibitory effects of the extracts were compared by relative maximum activity values (V_max) using proteases such as α-chymotrypsin, trypsin and papain, tyrosinase and β-lactamase (penicillinase).

• Antiproliferation
• enzyme inhibition
• Hep3B
• hop female cones
• HT-29
• Humulus lupulus
• proteases
• tyrosinase
• β-lactamase (penicillinase)

Acknowledgements

We acknowledge Gebze Institute of Technology and Balıkesir University for providing the laboratory facilities. We thank Bilge Şener and Hulusi Malyer for their help in this work.

Declaration of interest

The authors declare no competing financial interest.

We acknowledge the Çanakkale Onsekiz Mart University for financial support (Research University Grant BAP 2010/224).

Supplementary material available online

Supplementary Figures S1–10