Acetylcholinesterase Inhibition by Two Phosphoric 4-Nitroanilides

Abstract

Two phosphoric 4-nitroanilides Z₂(O)NH-ϕ-NO₂ (A, Z = Me; B, Z = NMe₂) have been prepared and purified by chromatographic techniques. Their spectral data (uv, ir and ¹H-nmr) have been determined, and compared with those of other similar compounds. Their ability to inhibit acetylcholinesterase has been measured by a modification of Ellman's method. The data, as computed according to the Michaelis scheme, indicate that A is not an inhibitor, whereas B is a reversible mixed one. These differences are discussed in terms of hydrophobic interactions.

Key Words: :

• Acetylcholinesterase
• Inhibition
• phosphoric anilides.