Inhibition of Adenylate Cyclase of Catfish and Rat Hepatocyte Membranes BY 9-(Tetrahydro-2-Furyl)Adenine (SQ 22536)

E. Fabbri Institute of General Physiology, University of Ferrara, Via L. Borsari, 46, 44100, Ferrara, Italy

L. Brighenti Institute of General Physiology, University of Ferrara, Via L. Borsari, 46, 44100, Ferrara, Italy

C. Ottolenghi Institute of General Physiology, University of Ferrara, Via L. Borsari, 46, 44100, Ferrara, Italy

Abstract

The adenosine analogue 9-(Tetrahydro-2-furyl)adenine, SQ 22536, inhibited adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] activity of crude membrane preparations from catfish (Ictalurus melas) and rat isolated hepatocytes in a non-competitive manner. The IC₅₀s were reduced in the presence of NaF. SQ 22536 reduced the activity of adenylate cyclase also in the presence of increasing concentrations of GTP, as well as Mg⁺⁺ and Mn⁺⁺. In the presence of catecholamines (epinephrine, norepinephrine, isoproterenol, phenylephrine) SQ 22536 reduced their activating effect on adenylate cyclase in both catfish and rat membranes. SQ 22536 also inhibited the effect of glucagon (0.1 UM) on rat membrane cyclase activity.

Key Words: :

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Inhibition of Adenylate Cyclase of Catfish and Rat Hepatocyte Membranes BY 9-(Tetrahydro-2-Furyl)Adenine (SQ 22536)

Information for

Open access

Opportunities

Help and information

Inhibition of Adenylate Cyclase of Catfish and Rat Hepatocyte Membranes BY 9-(Tetrahydro-2-Furyl)Adenine (SQ 22536)

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature