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Abstract
Objective. The aim of this work was to characterize the individual contribution of the amnion (AMN) and choriodecidua (CHD) regions to the secretion of human beta defensins (HBD)-1, -2, and -3, after stimulation with Streptococcus agalactiae.
Methods. Full-thickness membranes were mounted on a Transwell device, constituted by two independent chambers; 1 × 106 CFU/ml of S. agalactiae were added to either the AMN or CHD face or to both. Secretion profiles of HBD-1, HBD-2, and HBD-3 to the culture medium were quantified by enzyme-linked immunosorbent sandwich assay (ELISA).
Results. Secretion profile of HBD-1 remained without significant changes; HBD-2 secretion level by the CHD increased 2.0 (2.73 ± 0.19 pg/μg) and 2.6 (3.62 ± 0.60 pg/μg) times when the stimulus was applied only to the CHD region and simultaneously to both compartments, respectively. The bacterial stimulation in the AMN induced a 2.0 times (2.06 ± 0.29 pg/μg) increase in this region. HBD-3 secretion level increased significantly in the CHD (15.65 ± 2.68 pg/μg) and the AMN (14.94 ± 1.85 pg/μg) only when both regions were stimulated simultaneously.
Conclusion. The stimulation of human fetal membranes with S. agalactiae induced a differential and tissue-specific profile of HBD-1, HBD-2, and HBD-3 secretion.
Acknowledgments
We thank Irma Elena Sosa Gonzalez from the Infectology and Immunology Department for providing all the microbiological methodology. We thank Ingrid Masher for editorial work of manuscript. The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.
Declaration of interest:
This work was supported by the National Council of Science and Technology of Mexico (CONACyT) Grant No. 61590 to VZC.