An experimental model of neonatal normocapnic hypoxia and resuscitation in Landrace/Large White piglets

Abstract

Objective: The aim of this study is to describe and evaluate an experimental model of neonatal normocapnic hypoxia and resuscitation. Methods: Ten male Landrace/Large White neonatal piglets were studied. Following anaesthesia and intubation, the animals were mechanically ventilated. Surgical procedures included catheterization of the right internal jugular vein and the carotid artery. After stabilization with 21% O₂, normocapnic hypoxia was induced by decreasing the inspired O₂ to 6–8%. When piglets developed bradycardia (heart rate < 60 beats/min), reoxygenation was initiated by administering 21% O₂. Arterial blood samples were taken during baseline, hypoxia and reoxygenation in order to measure interleukine-6 and interleukine-8. Results: Nine out of ten animals were successfully resuscitated (one of these required chest compressions and a dose of adrenaline) and one died despite resuscitation efforts. After returning to baseline haemodynamic values, euthanasia was performed using thiopental overdose. Conclusions: Haemodynamic fluctuations at baseline, during normocapnic hypoxia and reoxygenation in Landrace/Large White piglets are comparable to that in human neonates, making the breed a favorable model of human neonatal hypoxia investigation.

Keywords::

• Neonatal hypoxia
• swine
• experimental model
• resuscitation

Acknowledgments

The authors wish to thank the Experimental-Research Center ELPEN Pharma where this experimental work was conducted.

Declaration of Interest: The authors report no declarations of interest