Proteomic analysis of midtrimester amniotic fluid to identify novel biomarkers for preterm delivery

Abstract

Objective: The aim of this study was to identify possible biomarkers for preterm delivery by analyzing midtrimester amniotic fluid. Methods: Thirty-two amniotic fluid samples were studied; 16 patients had a spontaneous preterm delivery and 16 patients delivered at term. The proteomic technique consisted of surface-enhanced laser desorption ionization time-of-flight (SELDI-TOF) using different types of solid chromatographic chips (Q10, CM10 and IMAC30). Results: Mass spectrometry tracings were obtained from the amniotic fluids of both patients who delivered preterm and patients who delivered at term. Seven potential markers were identified to be differentially expressed in patients who delivered preterm. Conclusions: Proteomic analysis of amniotic fluid obtained in the midtrimester reveals the presence of a set of proteins in patients at risk for preterm delivery.

Keywords::

• Amniotic fluid
• proteomics
• SELDI-TOF
• subclinical inflammation

Acknowledgements

The authors would like to thank medical laboratory technologist Fr. Korlach for her excellent technical assistance.

Declaration of Interest: The present study was approved by the Ethic Committee of the Charite University Hospital, date of approval 04.07.2003, Ref. Nr. 2003-322.