Effectiveness of strict anti-contamination criteria for non-invasive foetal sex determination in a clinical setting

Abstract

Objective: The aim of this study is to prevent false positive results in non-invasive foetal sex determination from pregnant women’s plasma following strict anti-contamination criteria.

Methods: cffDNA from 200 pregnant women (mean gestational age of 8.3 ± 0.2 weeks) was analyzed based on genetic forensic anti-contamination procedures and classical non-invasive foetal sex determination techniques.

Results: No false positive or false negative was reported. The sensitivity and the specificity reached both a 100% value.

Conclusions: Prevention of contamination following our anti-contamination criteria is a good practice for certain non-invasive sex determination, using cffDNA.

• Cell-free foetal DNA
• false positive
• non-invasive prenatal diagnosis
• real-time PCR
• reliability