Effects of sinusoidal electromagnetic fields on histopathology and structures of brains of preincubated white leghorn chicken embryos

Abstract

There are several reports indicating linkages between exposures to 50–60 Hz electromagnetic fields and abnormalities in the early stages of chicken embryonic development. Based on our previous published research carried out at the Department of Animal Sciences, Faculty of Biological Sciences, Shahid Beheshti University, effects of sinusoidal electromagnetic fields on histopathology and structures of brains of preincubated white leghorn hen eggs were investigated. Three hundred healthy fresh fertilized eggs (55–65 gr) were divided into three groups of experimental (n = 50), control (n = 75), and sham (n = 75). Experimental eggs (inside the coil) were exposed to 3 different intensities of 1.33, 2.66, and 7.32 mT and sham groups were located inside the same coil with no exposure, for 24 h before incubation. Control, sham, and experimental groups were all incubated in an incubator (38 ± 0.5^°C, 60% humidity) for 14 days. 14-day old chicken embryos were removed by C-sections, and the brains of all embryos of all groups were fixed in formalin(10%), stained with H&E and TUNEL assay, for studying the histopatholog and process of apoptosis. The brains of other embryos were prepared for Scanning Electeron Microscope. Results showed electromagnetic fields have toxic effects on brain cells by increasing the number of apoptotic cells and degeneration of brains' tissues of exposed chicken embryos. These findings suggest that the electromagnetic fields induce brain damages at different levels.

Keywords::

• EMF
• Chicken embryo
• Brain
• SEM
• Histopathology
• Apoptosis

Acknowledgements

The authors are grateful to Miss F. Moradi, Mr. A.Kalantari, and Shahid Behehshti University Medical Sciences Center of TEM and SEM for their unconditional cooperation.

Declaration of interestThe authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article.