Particle Electrophoresis of Compact Bone Tissue

Abstract

Fresh bovine bone, obtained from the diaphysis of a femur and a tibia, was used to determine the isoelectric point of bone particles. Particle electrophoresis equipment with a flat cell was used with a four electrode configuration. The results indicated that bone has an isoelectric point near pH 5 and that the overall electrophoretic mobility of the bone particles was similar to that of collagen particles obtained from human Achilles tendon.