In Vivo Effects of Millimeter Waves on Cellular Immunity of Cyclophosphamide-Treated Mice

Abstract

Reactions of peritoneal macrophages (PM) and T lymphocytes of BALB/c mice to in vivo exposure to millimeter (MM) waves were studied. The mice were additionally treated with the antineoplastic drug cyclophosphamide (CPA) 100 mg/kg. Phagocytosis of Candida albicans cells by PM obtained from mice treated with CPA and MM waves, alone and in combination, was studied in vitro using a monolayer technique. Proliferation of murine T cells (splenocytes) was studied by ³H-thymidine incorporation into DNA of T lymphocytes without stimulation and after stimulation with anti-CD3 antibody 2C11. The results of this study show that 61.22 GHz MM waves with an incident power density of 15 mW/cm² and a peak SAR of 420 W/kg significantly reduce the toxic effects of CPA on cellular immunity of mice in vivo as observed by the increased PM phagocytic activity and enhancement of T-cell proliferation.

Key Words:

• Antitoxic effects
• Cyclophosphamide
• BALB/c mice
• Millimeter waves
• Peritoneal macrophages
• T lymphocytes