Toxicological potential of mycotoxin MT81 and its benzoylated derivative on testicular spermatogenesis and steroidogenesis in mature male Wistar albino rats

Abstract

Mycotoxin MT81 was isolated, purified, and identified from a fungal strain of Penicillium nigricans. It is a CNS depressant, hyperglycemic agent and produces massive bone marrow depression, hepatotoxicity, and nephrotoxicity. Its benzolylated analog (benzoylated-MT81) was synthesized in our laboratory having a LD₅₀ value of 87.1 mg/kg body weight in mice. This study was designed to assess the toxicological effects of mycotoxin MT81 and its analog on testicular spermatogenesis and steroidogenesis in mature albino rats. The sperm count and percentage of motile sperm were decreased markedly in MT81- and benzoylated-MT81-treated rats. The body weight and the weight of testis were reduced, whereas weight of adrenal gland was increased in a dose-dependent manner in the toxin-treated rats. MT81 and its derivative caused accumulation of ascorbic acid and total cholesterol in the testis and reduction in the activities of Δ⁵-3β-hydroxysteroid dehydrogenase (Δ⁵-3β-HSD) and glucose-6-phosphate dehydrogenase (G-6-P-D), whereas the ascorbic acid and cholesterol content of adrenal gland were decreased and enzyme activities were elevated. This experiment suggests that MT81 and benzoylated-MT81 both produce inhibition of testicular spermatogenesis and steroidogenesis but increase adrenal steroidogenesis and ultimately sterility of male rats.

Keywords::

• MT81 and benzoylated-MT81
• sperm count
• percentage of motile sperm
• Δ⁵-3β-hydroxysteroid dehydrogenase
• glucose-6-phosphate dehydrogenase
• testicular spermatogenesis and steroidogenesis

Acknowledgement

The authors are grateful to ICMR, New Delhi, India for providing financial assistance.

Declaration of interest

The authors declares no conflict of interest.