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Abstract
Enzyme substrate-based analysis of canine aqueous humor was used to determine plasminogen activator inhibitor (PAI) activity. Commercially available, antibody-based methods for detection of PAIs are not cross-reactive with canine fibrinolytic mediators. Substrate-based enzymatic assays are not dependent upon the cross-reactivity of a primary antibody, but rather on specific enzyme-substrate interactions. The mean value (±1 SD) for PAI concentration in aqueous humor of dogs was 4.2 ± 1.1 IU/ml (n = 20). The median value for PAI concentration was 4 IU/ml. The distribution of PAI values was positively skewed with a range of values from 2.7 to 6.9 IU/ml. There was no significant difference (p > 0.05) between the mean PAI concentrations determined for male and female dogs.