Molecular and Cytogenetic Analysis of a Cerebellar Primitive Neuroectodermal Tumor with Prominent Neuronal Differentiation: Detection of MYCN Amplification by Differential Polymerase Chain Reaction and Southern Blot Analysis

Venita Jay Department of Pathology, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada

Jeremy Squire Department of Pathology, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada

Maria Zielenska Department of Pathology, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada

Blair Gerrie Department of Pathology, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada

Robin Humphreys Department of Neurosurgery, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada

Abstract

Oncogene amplification is uncommon in cerebellar primitive neuroectodermal tumor (medullo-blastoma) and its frequency and diversity are greater in medulloblastoma cell lines. We describe a medulloblastoma in a 10-year-old-girl with striking neuronal differentiation evident in the islands of ganglion cells intermixed with more primitive undifferentiated cells. The islands of ganglion cells showed prominent synaptophysin positivity. Karyotypic analysis revealed hypo -and hyperdiploidy with multiple random rearrangements and double minute chromosomes. Differential polymerase chain reaction and Southern blot analysis revealed up to 25-fold MYCN amplification.

Key Words:

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Molecular and Cytogenetic Analysis of a Cerebellar Primitive Neuroectodermal Tumor with Prominent Neuronal Differentiation: Detection of MYCN Amplification by Differential Polymerase Chain Reaction and Southern Blot Analysis

Information for

Open access

Opportunities

Help and information

Molecular and Cytogenetic Analysis of a Cerebellar Primitive Neuroectodermal Tumor with Prominent Neuronal Differentiation: Detection of MYCN Amplification by Differential Polymerase Chain Reaction and Southern Blot Analysis

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature