Synthesis and purification of the aflatoxin B₁-lysine adduct

Daiane Cristina SassDepartment of Food Engineering, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga, SP, Brazil,

Alessandra Vincenzi JagerDepartment of Food Engineering, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga, SP, Brazil,

Fernando Gustavo ToninDepartment of Biossystem Engineering, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga, SP, Brazil, and

Roice Eliana RosimDepartment of Food Engineering, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga, SP, Brazil,

Mauricio Gomes ConstantinoDepartment of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil

Carlos Augusto Fernandes OliveiraDepartment of Food Engineering, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga, SP, Brazil, Correspondence[email protected]

Abstract

This work reports the chemical synthesis of aflatoxin B₁ (AFB₁)-lysine based on procedures available in the literature, but using lysine without a protection group in the α-amine group. AFB₁-exo-8,9-epoxide was obtained by epoxidation of AFB₁ with chloroperoxybenzoic acid in dichloromethane and phosphate buffer. Purification and identification of the AFB₁-lysine were conducted by liquid chromatography (LC), and its structure was confirmed by LC with mass spectrometer and diode-array detection. The preparation of AFB₁-lysine using lysine without a protection group in the α-amine group was completed in 24 h, being a practical modification of available methods that can be reproduced in analytical laboratories.

Keywords

Declaration of interest

The authors report no conflicts of interest.

The authors thank the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for financial support and fellowships.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Synthesis and purification of the aflatoxin B₁-lysine adduct

Information for

Open access

Opportunities

Help and information

Synthesis and purification of the aflatoxin B1-lysine adduct

Abstract

Declaration of interest

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature

Synthesis and purification of the aflatoxin B₁-lysine adduct