Distribution of silver nanoparticles in pregnant mice and developing embryos

Carlye A. Austin Department of Biological Sciences, George Washington University, Washington DC, USA

Thomas H. Umbreit Center for Devices and Radiological Health, US Food & Drug Administration, Silver Spring MD, USA

Ken M. Brown Department of Biological Sciences, George Washington University, Washington DC, USA

David S. Barber Center for Environmental and Human Toxicology, University of Florida, Gainesville FL, USA

Benita J. Dair Center for Devices and Radiological Health, US Food & Drug Administration, Silver Spring MD, USA

Sabine Francke-Carroll Center for Food Safety and Nutrition, US Food & Drug Administration, College Park MD, USA

April Feswick Center for Environmental and Human Toxicology, University of Florida, Gainesville FL, USA

Melissa A. Saint-LouisReproductive Endocrinology and Fertility Department, Medical Faculty Associates, George Washington University, Washington DC, USA

Hiroyuki HikawaDepartment of Statistics, George Washington University, Washington DC, USA

Kerry N. SiebeinDepartment of Materials Science and Engineering, University of Florida, Gainesville FL, USA

Peter L. Goering Center for Devices and Radiological Health, US Food & Drug Administration, Silver Spring MD, USACorrespondence[email protected]

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Abstract

The objective of this study was to evaluate the distribution of silver nanoparticles (NPs) in pregnant mice and their developing embryos. Silver NPs (average diameter 50 nm) were intravenously injected into pregnant CD-1 mice on gestation days (GDs) 7, 8, and 9 at dose levels of 0, 35, or 66 μg Ag/mouse. Mice were euthanised on GD10, and tissue samples were collected and analysed for silver content. Compared with control animals injected with citrate buffer vehicle, silver content was significantly increased (p < 0.05) in nearly all tissues from silver NP-treated mice. Silver accumulation was significantly higher in liver, spleen, lung, tail (injection site), visceral yolk sac, and endometrium compared with other organs from silver NP-treated mice. Furthermore, silver NPs were identified in vesicles in endodermal cells of the visceral yolk sac. In summary, the results demonstrated that silver NPs distributed to most maternal organs, extra-embryonic tissues, and embryos, but did not accumulate significantly in embryos.

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Declarations of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

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Distribution of silver nanoparticles in pregnant mice and developing embryos

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Distribution of silver nanoparticles in pregnant mice and developing embryos

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