Discrete nanoparticles induce loss of Legionella pneumophila biofilms from surfaces

Abstract

Nanoparticles (NPs) have been shown to induce dispersal events in microbial biofilms but the mechanism of the dispersal is unknown. Biofilms contaminate many man-made aquatic systems such as cooling towers, spas and dental lines. Within these biofilms, Legionella pneumophila is a primary pathogen, leading to these environments serving as sources for disease outbreaks. Here we show a reduction in biofilm bio-volume upon treatment with citrate-coated 6-nm platinum NPs, polyethylene glycol (PEG)-coated 11-nm gold NPs, and PEG-coated 8-nm iron oxide NPs. Treatment with citrate-coated 8-nm silver NPs, however, did not reduce biomass. The synthesis of NPs that remain dispersed and resist irreversible aggregation in the exposure media appears to be a key factor in the ability of NPs to induce biofilm dispersal.

Keywords::

• nanoparticles
• biofilms
• gold
• iron oxide
• dispersal

Acknowledgements

The authors would like to thank Dr. Terri Bruce of the Jordan Hall Imaging Facility, Clemson University, for assistance with confocal microscopy, and Dan D'Unger for his help with nanoparticle synthesis. The work of Steven Saville and Bin Qi was supported by NSF grant DMR-0907167. This material is also based upon work supported by NIFA/USDA, under project number SC-1700463, Technical Contribution No. 6086 of the Clemson University Experiment Station.