Use of ¹⁸F-fluoride PET to determine the appropriate tissue sampling region for improved sensitivity of tissue examinations in cases of suspected periprosthetic infection after total hip arthroplasty

Abstract

Background and purpose The accurate diagnosis of periprosthetic infection requires assessment of intraoperative tissues. These must be sampled from the appropriate sites.

We used ¹⁸F-fluoride positron emission tomography (PET) to identify sites of inflammation in order to improve the sensitivity of histopathology, microbiological culture, and real-time PCR in total hip arthroplasty (THA) patients.

Patients and methods 23 THA patients (23 hips) scheduled for revision surgery (the revision group) and 17 uninfected THA patients (23 hips; control group) were enrolled. Uptake was classified into major, minor, and no uptake. To evaluate the association between the ¹⁸F-fluoride uptake and intraoperative tissue results in the revision group, we calculated their sensitivity on each of the major, minor, and no-uptake sides.

Results 17 revision patients showed major uptake and all were diagnosed as having septic loosening from intraoperative tissue results. Minor uptake was observed in the other 6 revision patients and all were diagnosed as having aseptic loosening. Apart from 3 cases that showed minor uptake regions, control subjects showed no uptake. In the revision group, the sensitivities of histopathology, microbiological culture, real-time PCR separately and also in combination were 0.78, 0.58, 0.96, and 0.96, respectively, on the major ¹⁸F-fluoride uptake sides, 0.0, 0.0, 0.1, and 0.1 on the minor-uptake sides, and 0, 0, 0.18, and 0.18 on the no-uptake sides.

Interpretation Our findings suggest that preoperative assessment of major uptake of ¹⁸F-fluoride markedly improves the accuracy of tissue sampling, and thus the sensitivity of subsequent tissue examinations. More definitive diagnosis of periprosthetic infection is therefore possible.

HC: design of the study, patient follow-up, surgery, PET investigations, pathology, PCR and preparation of the manuscript. YI: design of the study, surgery, preparation of the manuscript, and supervision. NK: design of the study, surgery, PET investigations, pathology, PCR, preparation of the manuscript, and supervision. HI, CA: patient follow-up. KS: design of the study and PET investigations. NI, YY, TIs: patient follow-up and surgery. TIn: design of the study, PET investigations, and supervision. TS: design of the study, preparation of the manuscript, and supervision.

We thank the following people for their contributions: Hiroshi Fujimaki, Taro Tezuka, Yasuhide Hirata, Takako Momose, Shusuke Fujiwara, all the orthopedic surgeons who participated, and the staff of the radiology and pathology departments at Yokohama City University.

No competing interests declared.