Abstract
Materials and methods: DNA sequences obtained for the Barcode of Life library in the All Lepidoptera Campaign project Nymphalidae of Central Mexico were analyzed as a test of species limits and to explore possible phylogenetic groupings in the Preponini tribe. Using specimens in the National Insect Collection of the Instituto de Biología of the Universidad Nacional Autónoma de México, 78 specimens were assayed for cytochrome oxidase c subunit 1.
Results: Disregarding the missing data, there were 458 conserved sites, 200 variable sites and 187 parsimony-informative sites. The neighbor-joining and maximum likelihood analyses indicate that none of the three genera of Preponini as currently circumscribed are reciprocally monophyletic. As per species limits, high levels of barcode variation in the Prepona deiphile complex suggest the existence of at least two new endemic species to Mexico. The divergent taxa were escalantiana from the Tuxtlas region in Veracruz, and ibarra from Sierra Madre del Sur in the Pacific states of southern Mexico. The genetic distance in the CO1 fragment between them and the other deiphile populations ranged from 2.7 to 8.0%.
Conclusion: We recommend that morphological data need to be re-examined and that additional molecular data for species ought to be gathered before a particular biogeographic model can be proposed for the group in Mesoamerica.
Acknowledgements
The authors thank Evgeny Zakharov, Marianne N. A. Iskandar, and Suresh Naik for laboratory work at the Biodiversity Institute of Ontario, University of Guelph. They also thank Matthew J. Miller, Postdoctoral Fellow at the Smithsonian Tropical Research Institute in Panama, for assistance with the analysis. Valuable advice was also received from Sergios-Orestis Kolokotronis and David Gernandt.
Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.