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Abstract
The complete mitogenome sequence of the spotted steed (Hemibarbus maculatus) was determined using long PCR reactions. The genome is 16,611 bp long and consists of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region. The gene order and composition of H. maculatus was similar to that of most other vertebrates. The base composition of H-strand in descending order is A (29.16%), C (27.41%), T (25.93%), and G (17.50%), with an AT content of 55.09%. The Kimura two-parameter distances between mitogenome sequences of H. maculatus and Hemibarbus labeo, Hemibarbus barbus, Hemibarbus longirostris, Hemibarbus mylodon were 2.09%, 2.35%, 12.77%, and 13.92%, respectively. The phylogenetic relationships among the five Hemibarbus species based on the whole mitogenome sequence data were (((H. barbus, H. labeo), H. maculatus), (H. longirostris, H. mylodon)).
Acknowledgment
The authors thank Lingli Yang and Jinjin Wang for the assistance with the laboratory work.
Declaration of interest: This work was supported by the grants from the National Basic Research (973) special preliminary study program (2010CB134405), the Special Fund for Agro-scientific Research in the Public Interest of China (200903048-08), and the National Natural Science Foundation of China (31071903). The authors report no conflict of interest. The authors alone are responsible for the content and writing of the paper.