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Abstract
The complete mitogenome sequence of a vulnerable species Onychostoma lini was determined using polymerase chain reaction and directly sequenced with primer walking method. The complete mitogenome was 16,595 bp in length, containing 37 genes of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region (D-loop). The gene order and composition of O. lini was similar to most other fishes. The descending order of the base composition on heavy strand was 31.6% A, 27.9% C, 24.6% T, and 15.9% G, with a relatively lower level of G and a slight AT bias of 56.2%. The codon usage followed the typical vertebrate mitochondrial pattern (ATG or GTG for start codon and TAA or TAG for stop codon). The complete mitogenome sequence of O. lini provides fundamental data for further conversation genetics and captive breeding studies on this species.
Acknowledgments
The authors thank Ping Li, Jinjin Wang, Dandan Xu, and Qing Zeng for their technical assistance.
Declarations of interest: This study was supported by the grants from the National Natural Science Foundation of China (31071903) and the Fundamental Research Funds for the Central Universities (XDJK2011B010). The authors declare no conflict of interest. The authors alone are responsible for the content and writing of the paper.