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Abstract
The long polymerase chain reaction and primer walking methods were employed for the complete mitochondrial genome (mitogenome) sequence of Japanese Konosirus punctatus. The mitogenome is a circular molecule 16,698 bp in length, including the typical structure of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 2 non-coding regions (L-strand replication origin and control region), the gene contents of which are identical to those observed in most bony fishes. Within the control region, we identified the termination-associated sequence domain, the central conserved sequence block domains (CSB-F, CSB-E, CSB-D, CSB-C, and CSB-B), and the conserved sequence block domains (CSB-2 and CSB-3).
Acknowledgements
We are grateful to Dr Hui Zhang and Dr Jiao Cheng for their helpful comments on the manuscript.
Declaration of interest: This study was supported by the Public Science and Technology Research Funds Projects of Ocean (201305030). The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.