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Abstract
The complete mitochondrial genome of the perlid stonefly Dinocras cephalotes (Curtis, 1827) was sequenced using a combined 454 and Sanger sequencing approach using the known sequence of Pteronarcys princeps Banks, 1907 (Pteronarcyidae), to identify homologous 454 reads. The genome is 15,666 bp in length and includes 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. Gene order resembles that of basal arthropods. The base composition of the genome is A (33.5%), T (29.0%), C (24.4%) and G (13.1%). This is the second published mitogenome for the order Plecoptera and will be useful in future phylogenetic analysis.
Acknowledgements
We would like thank Ralph Tollrian and Lars Dietz (Ruhr University Bochum) for support with this study and the members of the EvoEco Journal Club for helpful suggestions that improved this manuscript.
Declaration of interest
The work was supported by a grant of the Dinter foundation to F.L. V.E. and F.L. are supported by a grant of the Kurt Eberhard Bode Foundation within the Deutsches Stfitungszentrum. For the 454 sequencing, financial support was provided by the PACES research program of the Alfred Wegener Institute Helmholtz-Zentrum for Polar and Marine Research.