Haplotypes and variable position detection in the mitochondrial DNA coding region encompassing nucleotide positions 10,716–11,184

Abstract

This study evaluates the mitochondrial noncoding regions by using the Sanger sequencing method for application in Forensic Science. FTA® Technology (FTA™ paper DNA extraction) was utilized to extract DNA. Portion of coding region encompassing positions from (10,716 to 11,184) amplified in accordance with the Anderson reference sequence. PCR products purified by EZ-10 spin column were then sequenced and detected using the ABI 3730 × L DNA Analyzer. A new polymorphic positions 10,750 and 10,790 that are described may be suitable sources in future for identification purpose. The data obtained can be used to identify variable nucleotide positions characterized by frequent occurrence, most promising for identification variants.

• Coding region
• encompassing nucleotide positions from 10,716 to 11,184
• Iraq
• mitochondrial DNA
• polymorphic positions

Acknowledgements

I sincerely wish to thank Prof. Isam for providing me the opportunity to work on this project. I am indebted to you for choosing the project, your enthusiasm for helping me and your patience and guidance as I progress to put this project together. I am thankful to you for helping me through the various analysis stages, and for providing helpful criticism and feedback throughout the writing process. I also would like to thank Dr. Khalifa from the Institution of medico-legal for all time put in to discuss the project and helping me to put the project together. I would also like to thank Zainab Al-Habubi from the Department Biology for her guidance and help in the laboratory work.

Declaration of interest

We declare that we have no conflicts of interest in the authorship or publication of this contribution.