Genetic variability among Syphacia obvelata isolates from laboratory mice in four different geographical locations of China revealed by sequence analyses of five mitochondrial genes

Abstract

Syphacia obvelata is a rodent nematode with high prevalence in laboratory mice. In the present study, we examined the genetic variability of S. obvelata from naturally infected laboratory mice in four different provinces, China. Five mitochondrial (mt) DNA regions, namely cytochrome c oxidase subunit 1 (pcox1), cytochrome b (pcytb), large subunit ribosomal RNA (prrnL) and NADH dehydrogenase subunits 1 and 5 (pnad1 and pnad5), were amplified separately from individual nematodes by PCR, and then sequenced directly. The size of the sequences of pcox1, pcytb, prrnL, pnad1 and pnad5 was 628 bp, 555 bp, 548 bp, 548 bp and 561 bp, respectively. While the intra-specific sequence variations within S. obvelata were 0–1.0% for pcox1, 0–1.6% for pcytb, 0–2.8% for prrnL, 0–2.0% for pnad1 and 0–1.8% for pnad5, the inter-specific sequence differences among members of the Oxyuridae were significantly higher, being 14.0–17.5% for pcox1, 27.5–32.9% for pcytb, 35.8–37.2% for prrnL, 22.2–26.8% for pnad1 and 22.3–25.2% for pnad5, respectively. Phylogenetic analyses based on combined sequences of four mt protein-coding genes, using Bayesian inference (BI), maximum likelihood (ML) and maximum parsimony (MP) methods, indicated that all of the S. obvelata samples grouped together with high statistical support, but samples from the same geographical origin did not always cluster together. These findings demonstrated the existence of low-level intra-specific variation in five mtDNA sequences among S. obvelata isolates from laboratory mice, but no obvious geographical distinction among S. obvelata isolates from laboratory mice in different geographic regions in China. These results provide basic information for further studies of systematics and population genetics of S. obvelata.

• Laboratory mice
• mitochondrial DNA
• phylogenetic analyses
• sequence variability
• Syphacia obvelata

Declaration of interest

The experiments comply with the current laws of the country in which the experiments were performed. The authors report no conflicts of interests. The authors alone are responsible for doing the research and writing the paper. This work was supported by the Fund for Imported Talents in Heilongjiang Bayi Agricultural University (XDB2013-42).