Complete mitochondrial genome of the pink bollworm Pectinophora gossypiella (Lepidoptera: Gelechiidae)

Abstract

Pectinophora gossypiella (Lepidoptera: Gelechiidae) is a key pest in many cotton-growing countries of the world. In this study, the complete mitochondrial (mt) genome of the pink bollworm P. gossypiella was determined, which is 15,202 bp in length (GenBank accession number: KM225795) containing 37 typical animal mitochondrial gene and an A + T-rich region. The gene order of P. gossypiella mtDNA was different from the insect ancestral gene order in the translocation of trnM, as shared by previously sequenced lepidopteran mtDNAs. The protein-coding genes (PCGs) have typical mitochondrial start codons ATN, with the exception of COI, Nad5, which uses the start codons CGA, GTT. Eight PCGs stop with complete termination codons (TAA), whereas five PCGs use incomplete stop codon T. All of the tRNA genes had typical cloverleaf secondary structures except for trnS1(AGN), in which the dihydrouridine (DHU) arm did not form a stable stem-loop structure. Like other insects, the control region is located between rrnS and trnM with a length of 309 bp and an A + T content of 94.8%, which is the most AT-rich region and comparatively simple, with little evidence of long tandem repeats, but harbors a conserved structure combining the motif ATAGA and a 18-bp poly-T stretch.

• Gelechiidae
• Lepidoptera
• mitochondrial genome
• Pectinophora gossypiella

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article. We thank Dr Peng Wan (Hubei Academy of Agricultural Sciences) for providing insects used in our studies. This work was supported by the earmarked fund for Modern Agro-industry Technology Research System (CARS-18-16) and the National Key Technology Research and Development Program of China (2012BAD19B05-003).