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Abstract
We determined the complete mitochondrial genome (mitogenome) sequence of Papilio demoleus (GenBank accession number KR024009) by long PCR and primer walking methods. The total length of mitochondrial DNA is 15,249 bp containing 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. The overall base composition of the genome is A (39.31%), T (41.57%), C (11.33%) and G (7.78%) with an A + T-rich region, similar to other invertebrate mitochondrial genomes. The start codon was mainly ATG in most of the mitochondrial protein-coding genes such as COII, ATP6, COIII, ND4, ND4L, Cob and ND1, while ATA for ND2, COI, ATP8, ND3, ND5 and ND6 genes. The stop codon was mainly TAA in most of the mitochondrial protein-coding genes, whereas TAG was found in ND1 gene only. The A + T region is located between 12S rRNA and tRNAMet with a length of 403 bp.
Declaration of interest
This study was supported by Natural Science Foundation of China (31460553, 31160418, 31160081), Natural Science Foundation of Jiangxi (2013BAB204005), Science & Technology Foundations of Education Department of Jiangxi (GJJ13097, GJJ13104, KJLD14014), National Training Programs of Innovation and Entrepreneurship for Undergraduates (20140403023) and Foundations from the Administration of Science & Technology in Nanchang city (2010NYZZ037, 2013HZCG008). The authors alone are responsible for the content and writing of the paper.