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Mitochondrial DNA Part A
DNA Mapping, Sequencing, and Analysis
Volume 27, 2016 - Issue 4
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Mitogenome Announcement

The complete mitochondrial genome sequence of Red knobby newt Tylototriton shanjing (Amphibia: Caudata)

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Pages 2773-2774 | Received 25 Apr 2015, Accepted 04 May 2015, Published online: 11 Jun 2015
 

Abstract

The complete mitogenome of Tylototriton shanjing is 16,661 bp in length with GenBank accession number KR154461, which contains 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNA), 2 ribosomal RNA genes (rRNA), and 1 control region (CR). The overall base composition of this mitogenome is biased toward AT content at 59.45%. Most of the PCGs and tRNA genes are located on the H-strand, except for ND6 subunit gene and eight tRNA genes, which were distributed on the L-strand. The PCGs used “ATG” and “GTG” as the start codons, while “TAA”, “TAG”, “AGA”, and “T–” are used as stop codons. Almost all tRNA genes were folded into typical cloverleaf secondary structures. The T. shanjing genome had two tandem repeat sequences in the cob-noncoding region. The mitogenomic phylogenetic analyses shows that the genera Echinotriton and Tylototriton were clustered into a strong supported monophyletic clade, which is a sister clade to the genus Pleurodeles, this confirms the previous phylogenetic results.

Acknowledgements

We are grateful to Dr. Guohui Yang, Pengfei Fan and Wen Xiao for their assistance during our field work. We also thank Mr. Jiabin Liu for valuable comments on the manuscript preparation.

Declaration of interest

The authors report that they have no conflicts of interest. This research was supported by the National Natural Science Foundation of China to M. W. Zhang (41470444), Foundation of Education Department of Sichuan to M. W. Zhang (13ZA0262), and the Innovative Research Team in University of Sichuan Bureau of Education (14TD0002).

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