Mitochondrial DNA Part A

DNA Mapping, Sequencing, and Analysis

Volume 27, 2016 - Issue 4

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Mitogenome Announcement

Ion torrent next-generation sequencing reveals the complete mitochondrial genome of endangered mahseer Tor khudree (Sykes, 1839)

Sudhanshu RamanDivision of Fish Genetics and Biotechnology, ICAR – Central Institute of Fisheries Education (Deemed University), Mumbai, India,

A. Pavan-KumarDivision of Fish Genetics and Biotechnology, ICAR – Central Institute of Fisheries Education (Deemed University), Mumbai, India, Correspondence[email protected]

Prakash G. KoringaDepartment of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India, and

Namrata PatelDepartment of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India, and

Tejas ShahDepartment of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India, and

Rajeev K. SinghICAR – National Bureau of Fish Genetic Resources, Dilkusha PO, Lucknow, India

Gopal KrishnaDivision of Fish Genetics and Biotechnology, ICAR – Central Institute of Fisheries Education (Deemed University), Mumbai, India,

C. G. JoshiDepartment of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India, and

P. Gireesh-BabuDivision of Fish Genetics and Biotechnology, ICAR – Central Institute of Fisheries Education (Deemed University), Mumbai, India,

Aparna ChaudhariDivision of Fish Genetics and Biotechnology, ICAR – Central Institute of Fisheries Education (Deemed University), Mumbai, India,

W. S. LakraDivision of Fish Genetics and Biotechnology, ICAR – Central Institute of Fisheries Education (Deemed University), Mumbai, India,

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Abstract

The complete mitochondrial genome of an endangered mahseer (Deccan mahseer), Tor khudree was sequenced using Ion torrent platform for the first time. The genome sequence was 16 573 bp in size, and consists of 13 protein coding genes, 22 tRNAs, 2 rRNA genes and 1 control region. The gene organization and its order were similar to other vertebrates. The overall base composition was A: 31.9%, G: 15.6%, C: 27.68%, T: 24.76%, A + T content 56.6% and the G + C content 43.32%. The phylogenetic tree constructed using a maximum likelihood model showed sister relationship between T. khudree and Tor tambroides.

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The authors report that they have no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

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Ion torrent next-generation sequencing reveals the complete mitochondrial genome of endangered mahseer Tor khudree (Sykes, 1839)

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Ion torrent next-generation sequencing reveals the complete mitochondrial genome of endangered mahseer Tor khudree (Sykes, 1839)

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