Genetic diversity and relationship of Mauremys mutica and M. annamensis assessed by DNA barcoding sequences

Abstract

The mitochondrial DNA cytochrome c oxidase subunit I gene (COI) has been used as an efficient barcoding tool for species identification of animals. In this study, the barcoding sequences were used to assess the genetic diversity and relationship of Mauremy mutica and M. annamensis. Four currently recognized groups of M. mutica were classified into two groups in this study, with 6% intergroup distances, the S group and the N group, consistent to the calling of “southern turtle” and “northern turtle” in folk of China. The north population and Taiwan population formed the N group, and further, the Taiwan population was differentiated as a monophyly originated from the north population, consistent to the calling of “big green head” for the Taiwan population and “small green head” for the north population. The Vietnam, Hainan population, and M. annamensis formed the S group, and the barcoding sequences could not distinguish them from each other. Based on the molecular data and phenotypes of existing hybrids, hybrid origin of M. annamensis may be another possibility.

• DNA barcoding
• genetic differentiation
• genetic diversity
• Mauremys annamensis
• Mauremys mutica

Declaration of interest

This research was funded by the National Science and Technology Basic Research Program of China (2013FY110700). Special Scientific Research Funds for Central Non-profit Institutes, Chinese Academy of Fishery Sciences (2013A0504 and 2014A11JC06) and National Natural Science Foundation of China (31302176).