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Abstract
Background: More than 50 ascidian species distributed in the Palk Bay, Southeast coat of India. Up to a very few molecular work has been performed to determine the evolutionary relationships of ascidians in the world. Objectives: Present study explored the value of mtDNA data in assessing phylogenetic relationships within the family Ascidiacea, Didemnidae, Styelidae, and molecular identification of ascidians from the Palk Bay, Southeast coast of India. Materials and methods: The phylogeny analysis was executed based on mitochondrial cytochrome oxidase subunit I (COI) sequences of ascidian species. A BLASTN search can be run to determine the similarity of an unknown DNA sequence (query) with the collection of all known DNA sequences in GenBank. Result: The BLASTN results showed that Didemnum candidum, Ascidia ahodori and Styela clava match the other listings for these species in GenBank. Mitochondrial COI gene sequences of collected ascidians were submitted to GenBank and obtained the accession numbers. Conclusion: This study is the first report of molecular identification of ascidian species of the Palk Bay, Southeast coast of India. This information about Palk Bay ascidian communities provides a baseline of general biodiversity of that ecosystem.
Acknowledgements
The authors thank the Dean and Director of CAS in Marine Biology and authorities of Annamalai University for providing the facilities.
Declaration of interest
The authors report no conflicts of interest. Authors gratefully acknowledge the financial assistance of a major research project (F.No.42-603/2013 (SR) dt. 22.03.2013) by the University Grants Commission, New Delhi, India.