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Abstract
Cannabis and Humulus are sister genera comprising the entirety of the Cannabaceae sensu stricto, including C. sativa L. (marijuana, hemp), and H. lupulus L. (hops) as two economically important crops. These two plants have been used by humans for many purposes including as a fiber, food, medicine, or inebriant in the case of C. sativa, and as a flavoring component in beer brewing in the case of H. lupulus. In this study, we report the complete chloroplast genomes for two distinct hemp varieties of C. sativa, Italian “Carmagnola” and Russian “Dagestani”, and one Czech variety of H. lupulus “Saazer”. Both C. sativa genomes are 153 871 bp in length, while the H. lupulus genome is 153 751 bp. The genomes from the two C. sativa varieties differ in 16 single nucleotide polymorphisms (SNPs), while the H. lupulus genome differs in 1722 SNPs from both C. sativa cultivars.
Acknowledgements
The authors thank Ben Holmes and Centennial Seeds for the plant donation, support, and help on this project, and Jacob K. Keller for annotating the Dagestani chloroplast.
Declaration of interest
This project was supported by the University of Colorado Foundation gift fund 13401977-Fin8, the University of Colorado’s Innovative Seed Grant Program grant to N. K., and the NSF IGERT Fellowship for Interdisciplinary Quantitative Biology (IQBiology) to K. G. K.