The High-throughput sequencing of Sillago japonica mitochondrial genome reveals the phylogenetic position within the genus Sillago

Abstract

The complete mitogenome of Sillago japonica was determined through high-throughput DNA sequencing technology. The circular mtDNA molecule was 16 645 bp in size and encoded 13 protein-coding genes, 2 rRNAs, 22 tRNAs and 2 non-coding regions, with the gene arrangement and content identical to other typical vertebrate mitogenomes. The identity analysis revealed that the mitogenome sequence of S. japonica shared a relatively high sequence identity to S. asiatica (81.5%) compared with S. aeolus (77.5%), S. indica (77.1%), and S. sihama (76.3%). The neighbor-joining tree of complete mitogenome sequence showed that S. japonica firstly clustered together with S. asiatica, then grouped with S. indica and S. sihama, and finally gathered with S. aeolus. Taken together, the results absolutely supported the evolutionary position of S. japonica and provided new insights into phylogenetic relationships of Sillago.

• High-throughput sequencing technology
• mitogenome, Sillago japonica

Declaration of interest

This study was financially supported by the Special Fund for Agro-scientific Research in the Public Interest of China (201403008), National Natural Science Foundation of China (31372532), Project for Outstanding Young Teachers in Higher Education of Guangdong, China (Yq2013093), and Fund of Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, the People's Republic of China (LSF2014-03). The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.