Novel Nanodimension artificial red blood cells that act as O₂ and CO₂ carrier with enhanced antioxidant activity: PLA-PEG nanoencapsulated PolySFHb-superoxide dismutase-catalase-carbonic anhydrase

Abstract

Poly(ethylene glycol)-Poly(lactic acid) block-copolymer (PEG-PLA) was prepared and characterized using Fourier transform infrared spectrophotometer (FTIR). Glutaraldehyde was used to crosslink stroma-free hemoglobin (SFHb), superoxide dismutase (SOD), catalase (CAT), and carbonic anhydrase (CA) into a soluble complex of PolySFHb-SOD-CAT-CA. PEG-PLA was then used to nanoencapsulated PolySFHb-SOD-CAT-CA by oil in water emulsification. This resulted in the formation of PLA-PEG-PolySFHb-SOD-CAT-CA nanocapsules that have enhanced antioxidant activity and that can transport both O₂ and CO₂. These are homogeneous particles with an average diameter of 100 nm with good dispersion and core shell structure, high entrapment efficiency (EE%), and nanocapsule percent recovery. A lethal hemorrhagic shock model in rats was used to evaluate the therapeutic effect of the PLA-PEG-PolySFHb-SOD-CAT-CA nanocapsules. Infusion of this preparation resulted in the lowering of the elevated tissue PCO₂ and also recovery of the mean arterial pressure (MAP).

Keywords::

• artificial
• cells
• carbon dioxide
• catalase
• carbonic anhydrase
• oxygen
• PLA-PEG nanocapsules
• PolySFHb-SOD-CAT-CA
• polyhemoglobin
• transport
• transfusion
• superoxide dismutase

Acknowledgements

Professor TMS Chang acknowledges the operating term grant from the Canadian Institute of Health Research (MOP13745) and Wei Gao's China Scholarship Council scholarship for her to come here as a PhD trainee. Jeffrey Chi Yung Tso, is a summer student who acted mostly as an observer.

Declaration of interest

The authors report no declarations of interst. The authors alone are responsible for the content and writing of the paper.