The effect of pretreatment with substrates on the activity of immobilized pancreatic lipase

Abstract

Porcine pancreatic lipase was covalently immobilized on polyvinyl alcohol using adipoyl dichloride as a cross-linker. The effect of pre-treatment of lipase previously with various types of oils on immobilization efficiency was investigated. The increment in immobilization efficiency was observed after pre-treatment of lipases with oils. The highest immobilization efficiency obtained was 20% (v/v) for olive oil pre-treated lipase, which was 8 times higher than that of non-pretreated immobilized lipase. Immobilized lipase had better stability and had some advantages in comparison with free enzyme.

Keywords::

• adipoyldichloride
• immobilization
• polyvinylalcohol
• porcine pancreatic lipase
• substrate pre-treatment

Declaration of interest

The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper. The authors gratefully acknowledge the support for this research provided by the Scientific Research Foundation of Ege University (BAP - Grant Number Sci 2007–011).