Fresh broad (Vicia faba) tissue homogenate-based biosensor for determination of phenolic compounds

Abstract

In this study, a novel fresh broad (Vicia faba) tissue homogenate-based biosensor for determination of phenolic compounds was developed. The biosensor was constructed by immobilizing tissue homogenate of fresh broad (Vicia faba) on to glassy carbon electrode. For the stability of the biosensor, general immobilization techniques were used to secure the fresh broad tissue homogenate in gelatin-glutaraldehyde cross-linking matrix. In the optimization and characterization studies, the amount of fresh broad tissue homogenate and gelatin, glutaraldehyde percentage, optimum pH, optimum temperature and optimum buffer concentration, thermal stability, interference effects, linear range, storage stability, repeatability and sample applications (Wine, beer, fruit juices) were also investigated. Besides, the detection ranges of thirteen phenolic compounds were obtained with the help of the calibration graphs. A typical calibration curve for the sensor revealed a linear range of 5–60 μM catechol. In reproducibility studies, variation coefficient (CV) and standard deviation (SD) were calculated as 1.59%, 0.64 × 10⁻³ μM, respectively.

Keywords::

• amperometric biosensor
• fresh broad
• phenolic compound
• tissue biosensor
• Vicia faba

Acknowledgements

We would like to thank Dr. E. Dinckaya, Dr. A. Telefoncu, Dr. M.K. Sezginturk and Dr. D. Odacı (Biochemistry Department, Faculty of Science, Ege University, •zmir/Turkey) for their support.

Declaration of interest

The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.

Financial support for this work was provided by Trakya University (Edirne, Turkey), throughout the projects TUBAB-843.