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Abstract
The present study was aimed at exploring the targeting potential of LTA-anchored chitosan nanoparticles (CH-NP) specifically to M cell following oral immunization. The lectinized CH-NP exhibited 7–29% coupling capacity depending upon the amount of glutaraldehyde added. Induction of the mucosal immunity was assessed by estimating secretory IgA level in the salivary, intestinal and vaginal secretions, and cytokine (IL-2 and IFN-γ) levels in the spleen homogenates. The results demonstrated that LTA-anchored CH-NP elicited strong humoral and cellular responses and hence could be a competent carrier-adjuvant delivery system for oral mucosal immunization against Hepatitis B.
Acknowledgement
Authors acknowledge Prof. S. C. Lakhotia, BHU, Varanasi, India for providing confocal facility and NIPER, Mohali for providing AFM facility. Authors also acknowledge Shantha Biotech Ltd. (Hyderabad, India) for providing recombinant hepatitis B surface antigen as gift sample.
Declaration of interest
The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.
The present work was supported by grants from Indian Council of Medical Research, New Delhi, India. One of the authors, Dr. Neeraj Mishra acknowledges ICMR- SRF (New Delhi, India) (Grant: 45/02/2007-BMS/PHA Dated 06\07\2007\H.S.Gour) for providing financial assistance.