Nanoplatforms attached Schiff bases by condensation method; Investigation of glucose oxidase enzyme as biocatalysts

Abstract

We report a easy approach for the immobilization of glucose oxidase enzyme (GOx) on nanoparticles. Nanoparticles-modified polystyrene-ANH₂ (PSA) with some salicylaldehyde derivatives were synthesized by means of condensation and investigated the enzymatic properties of GOx immobilized on there. Modified polystyrenes were characterized using IR spectra, gel permeation chromatography, and scanning electron microscopy. All the characteristics of the immobilized glucose oxidase (PSA-SalH)-GOx, (PSA-SalCH₃)-GOx, and (PSA)-GOx enzyme showed except one of them. Immobilized GOx on to (PSA) showed two optimum pH, other immobilized polymers were one optimum.

Keywords::

• biocatalysis
• salicylaldehyde
• glucose oxidase
• nanoparticle

Declaration of interest

The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.

This work was supported by the Gazi University Research Fund (Project number: 05/2010-75 and 05/2012-68).