The effect of TGF-β1 and BMP-4 on bone marrow-derived stem cell morphology on a novel bioabsorbable nanocomposite material

Abstract

Context: Congenital heart disease is a leading cause of death in the first year, with an incidence of 1.5 million worldwide. It can be treated with bypass surgery, but due to the limited availability of autologous grafts, there has been research into developing a completely tissue-engineered vascular graft. Our group has developed a small diameter, biodegradable nanocomposite graft which is non-thrombogenic and biostable. Objectives: This study looks at the effects of the growth factors, TGF-β1 and BMP-4 on bone marrow-derived stem cell (BMSC) morphology on a POSS PCL scaffold. Materials and methods: BMSCs were seeded onto a new nanocomposite of POSS (polyhedral oligomeric silsesquioxane) and PCL (poly[caprolactone-urea]urethane) and TGF-β1 and BMP-4 were used to induce differentiation of the cells to smooth muscle cells. The distribution of the cells was examined using confocal and electron microscopies and the phenotype of the cells was assessed using immunohistochemistry. Results: It was found that growth factor induction led to a decrease in cell growth on POSS PCL as compared to that of the control surface, and confocal microscopic analysis showed less cytoskeleton reorganization of these cells. After immunohistochemistry analysis, the BMSCs showed no differentiation to smooth muscle cells. Discussion: Growth factor induction on the static scaffold discs led to a change in morphology, with less spreading of the cells, a lower proliferation rate and no differentiation into SMCs. These findings can be attributed to the POSS PCL being manufactured by a coagulation technique, resulting in a structure with low stiffness.

Keywords::

• bypass graft
• congenital heart disease
• growth factors
• smooth muscle cells
• tissue engineering

Acknowledgements

I would like to thank Dr Achala de Mel and Dr Maria Manikou for their support in the lab, and also Arnold for his support in the polymer lab.

Declaration of interest

The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.