Ex vivo localization and permeation of cisplatin from novel topical formulations through excised pig, goat, and mice skin and in vitro characterization for effective management of skin-cited malignancies

Abstract

Context: It would be advantageous to administer cisplatin topically for treatment of cutaneous malignancies. Objectives: Present work focuses on ex vivo and in vitro characterization of proultraflexible topical formulations. Materials and methods: Permeation of cisplatin through the excised pig, goat, and mice skin was quantitatively determined. Results: Data indicate that protransfersome carbopol gel (pcg) formulation clearly delayed drug permeation through skin. Permeation of cisplatin from protransfersome system (ps) formulation was enhanced by approximately 1.5 fold compared with pcg for pig and goat skin. Discussion: Localization of drug from pcg was higher and showed less permeation. Conclusion: Cisplatin-loaded pcg formulation is better to treat cutaneous malignancies.

Keywords::

• A-431 cell line
• cutaneous squamous cell carcinoma
• cytotoxic activity
• fluorescence microscopy
• local accumulation efficiency
• proultraflexible topical formulations

Acknowledgment

The authors would like to express their gratitude to the National Institute of Cell Sciences for providing cell line and, Prof (Dr) Kishore G Bhat, Dept of Microbiology, Maratha Mandal's NGH Institute of Dental Sciences & Research Centre, Belgaum, Karnataka, for providing in vitro anticancer activity. The authors are grateful to Department of Science and Technology (DST), Govt. of India, New Delhi for financial support under Women Scientist Scheme (WOS-A) grant for research work.

Declaration of interest

The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.