Preparation and evaluation of multifunctional envelope-type nano device loading siRNA

Abstract

We prepared multifunctional envelope-type nano device (MEND) loading siRNA through lipid film hydration method; the qualities of MEND were characterized by the shape, particle size, and evaluated by the encapsulation efficiency (EE), plasma stability, and transfection efficiency. The formulated MEND was found to be relatively uniform in size with a positive zeta potential. The average siRNA entrapment efficiency was 86.4%. After 48 h exposure in 50% (v/v) serum at 37°C, the siRNA in MEND had no significant degradation. When the concentration of siRNA in 24-well plates was 500 nM, the MEND had high transfection efficiency, almost 100%.

Keywords::

• multifunctional envelope-type nano device
• non-viral vector
• orthogonal test
• siRNA
• transfection

Declaration of interest

The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.

This work was supported by the National Natural Science Foundation in 2014 (81373350) and Qingdao Science & Technology project in 2013 (13-1-3-49-nsh).