Surface-modified bacterial nanofibrillar PHB scaffolds for bladder tissue repair

Abstract

The aim of the study is in vitro investigation of the feasibility of surface-modified bacterial nanofibrous poly [(R)-3-hydroxybutyrate] (PHB) graft for bladder reconstruction. In this study, the surface of electrospun bacterial PHB was modified with PEG- or EDA via radio frequency glow discharge method. After plasma modification, contact angle of EDA-modified PHB scaffolds decreased from 110 ± 1.50 to 23 ± 0.5 degree. Interestingly, less calcium oxalate stone deposition was observed on modified PHB scaffolds compared to that of non-modified group. Results of this study show that surface-modified scaffolds not only inhibited calcium oxalate growth but also enhanced the uroepithelial cell viability and proliferation.

Keywords::

• bladder reconstruction
• calcium oxalate stones
• nanotexture
• poly [(R)-3-hydroxybutyrate] (PHB)
• tissue engineering

Acknowledgments

This study was carried out under the auspices of the Hacettepe University, Scientific Researches Unit.

Declaration of interest

The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.