A novel glutamine biosensor based on zinc oxide nanorod and glutaminase enzyme from Hypocria jecorina

Abstract

A novel biosensor for determination of L-glutamine in pharmaceutical glutamine powder was developed via immobilizing our produced glutaminase enzyme from Hypocria jecorina onto our prepared zinc oxide (ZnO) nanorod and chitosan. ZnO nanorods were prepared as surface-dependent and surface-independent and both were used. The biosensor is specific for L-glutamine and the peculiar analytical properties (linearity range, reproducibility, and accuracy) of it were experimentally determined. The optimum operating conditions of the biosensor such as buffer concentration, buffer pH, and medium temperature effect on the response of biosensor were studied. K_m and V_max values for the our-producing glutaminase enzyme from Hypocria jecorina immobilized on the biosensor were also determined as 0.29 mM and 208.33 mV/min., respectively, from Lineweaver–Burk plot. The biosensor was then used for the determination of glutamine contained in pharmaceutical formulations.

Keywords::

• biosensor
• chitosan
• glutamine
• metal oxide nanorod
• nanorod
• zinc oxide

Declaration of interest

The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.

We gratefully acknowledge for the financial support of Yildiz Technical University Science Research Project Foundation (Project No: 2012-01-02-KAP10).