Gene silencing effect of SiRNA-magnetic modified with biodegradable copolymer nanoparticles on hTERT gene expression in lung cancer cell line

Abstract

Background

Telomerase is expressed in most of malignant cells, including lung cancer cells. The success of small interfering RNA (siRNA) in silencing of the telomerase catalytic subunit depends upon carriers ability to efficiently deliver therapeutic agent to cells with minimal toxicity and most biocompatibility. In this study, a potential carrier for efficient delivery was assessed by siRNA encapsulating into Iron MNPs modified with biodegradable polyester nanoparticles consisting of PLGA and PEG.

Results

Data analysis shows that the self-assemble diblock copolymers were synthesized, and then the siRNA designed against hTERT catalytic subunit was encapsulated. Also, the rate of telomerase gene expression in equivalent with magnetic copolymers/siRNA was lower than that of free siRNA (P = 0.001).

Conclusions

In conclusion, regarding the enhancing of siRNA stability by magnetic copolymer, the expression of telomerase gene was significantly lower in the cells treated with siRNA-magnetic copolymers than those treated with free siRNA.

Keywords::

• gene silencing
• lung cancer
• nanoparticles
• SiRNA delivery
• telomerase

Authors’ contributions

AA conceived of the study and participated in its design and coordination. NZ participated in the sequence alignment and drafted the manuscript. All authors read and approved the final manuscript.

Acknowledgments

The authors thank Department of Medical Nanotechnology, Faculty of Advanced Medical Science of Tabriz University for all supports provided.

Declaration of interest

The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.

This work is funded by Lung Research Center of Tabriz University of Medical Sciences, Tabriz.