Genetically modified luminescent bacteria Ralostonia solanacerum, Pseudomonas syringae, Pseudomonas savastanoi, and wild type bacterium Vibrio fischeri in biosynthesis of gold nanoparticles from gold chloride trihydrate

Abstract

The effect of different genetically engineered bacteria, Pseudomonas syringae, Pseudomonas savastanoi, and Ralostonia solanacerum and also a natural marine bacterial species, Vibrio fischeri NRRL B-11177, is studied in producing gold nanoparticles. This is the first report about the biosynthesis of gold nanoparticles by natural and genetically engineered luminescent bacteria. These microorganisms reduced gold ions and produced fairly monodisperse nanoparticles. TEM analysis indicated that spherical nano gold particles in the different diameters and shapes were obtained at pH values of 6.64. In this biosynthesis protocol, the gold nanoparticles with desired shape and size can be prepared.

Keywords::

• biological synthesis
• genetically engineered bacteria
• gold ions
• nano gold particles
• plant pathogens
• reduction reaction
• surface plasmon absorption band
• transmission electron microscopy

Declaration of interest

The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.

We are thankful to the Ferdowsi University Research Council for the financial support of this work (Grant P 53:24-01-89).