An amperometric biosensor for L-glutamate determination prepared from L-glutamate oxidase immobilized in polypyrrole-polyvinylsulphonate film

Abstract

In this paper, a novel amperometric L-glutamate (Glu) biosensor with immobilization of L-glutamate oxidase (L-GlOx) on polypyrrole-polyvinylsulphonate (PPy-PVS) film has been successfully developed. L-GlOx enzyme was immobilized on PPy-PVS film by cross-linking with glutaraldehyde (GA) and bovine serum albumin (BSA). Determination of Glu was carried out by oxidation of enzymatically produced H₂O₂ at 0.3 V versus Ag/AgCl. The optimum pH and temperature parameters were found to be 9.0 and 55°C, respectively. There were three linear parts in the regions between 1.0 × 10^{− 9} and 1.0 × 10^{− 8} M (R² = 0.847), 5.0 × 10^{− 8} and 5.0 × 10^{− 7} M (R² = 0.997), 5.0 × 10^{− 7} and 5.0 × 10^{− 5} M (R² = 0.994). Storage stability, operation stability of the enzyme electrode were also studied.

Keywords::

• biosensor
• L-Glutamate
• L-glutamate oxidase
• polypyrrole
• polyvinylsulphonate

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

The authors are very grateful for the financial support of Gazi University Research Fund (Project no: 05/2013-05).