A new PANI biosensor based on catalase for cyanide determination

Abstract

Cyanide is one of the most widespread of compounds measured in environmental analysis due to their toxic effects on environment and health. We report a highly sensitive, reliable, selective amperometric sensor for determination of cyanide, using a polyaniline conductive polymer. The enzyme catalase was immobilized by electropolymerization. The steps during the immobilization were controlled by electrochemical impedance spectroscopy. Optimum pH, temperature, aniline concentration, enzyme concentration, and the number of scans obtained during electropolymerization, were investigated. In addition, the cyanide present in artificial waste water samples was determined. In the characterization studies of the biosensor, some parameters such as reproducibility and storage stability, were analyzed.

Keywords::

• catalase
• cyanide
• electrochemical impedance spectroscopy
• inhibitory biosensor
• PANI

Declaration of interest

The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.