Separation and determination of reduced vitamin C in polymerized hemoglobin-based oxygen carriers of the human placenta

Abstract

The molybdenum blue method was used to determine the content of reduced vitamin C (Vc) in a solution of polymerized hemoglobin-based oxygen carriers (HBOCs) of the human placenta. The conditions of absorption wavelength, HCl addition, and reaction time, were investigated. The results of validation experiments showed that under the optimized conditions, a standard curve was confirmed with good linearity of 0.9985, for the Vc amount ranging from 0–200 μg. The values for relative standard deviation (RSD) of the precision and repeatability were both below 5%. Vc recovery was in the range of 97–102%. The conclusion could be made that a reduction in Vc content could be tested effectively by the molybdenum blue method.

Keywords::

• hemoglobin-based oxygen carriers
• molybdenum blue method
• spectrometry
• Vitamin C

Declaration of interest

The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.

The authors are grateful to the financial support from the National High Technology Research and Development Program (863 Program) of China (No.2012021903-3), the Science and Technology support program of Sichuan Province in China (No.2013SZ0059), and the Innovation project of Sichuan Province in China (No.2013ZZ0006).